How to see the report of renal puncture?
summary
Renal biopsy for histopathological examination is the most commonly used diagnostic means of kidney disease. It is not only used in the pathological diagnosis of autogenous kidney or transplanted kidney, but also helps to further understand the occurrence, development and prognosis of the disease, and provides more information for guiding treatment and judging prognosis. How do we look at it? Let's share some tips.
How to see the report of renal puncture?
First, immunofluorescence antibody technology is based on the principle that an antibody can only specifically bind to the corresponding antigen. Fluorescein is labeled on the known antibody, and the known antibody labeled with fluorescein reacts with the kidney tissue to be examined. If there is an antigen corresponding to the known antibody in the kidney tissue, the two will combine to form an immune complex of the known antibody fluorescein, The complex was observed under a fluorescence microscope with ultraviolet light source, and fluorescein, as an indicator, was excited to emit bright fluorescence, which indicated that there was corresponding antigen in the part where the intrinsic fluorescence appeared. Second, immunofluorescence is an indispensable method in the pathological examination of glomerular diseases. It should be noted that the known types of antibodies and complements should be complete, and there should be 1-2 glomeruli.
Third: globulin: the human immunoglobulin (IgG, IgA, IgM) in renal tissue section was routinely examined by immunofluorescence technique to determine the types of globulin involved in the immune response; Complement: such as C3, C1q, in order to confirm the pathway of complement activation in the process of immune response, namely classical or bypass pathway
matters needing attention
1. Please clean your skin before operation, such as taking a shower or wiping your body. 2. Relax your mind before operation to avoid emotional tension. If you can't sleep at night, please inform the nurse on duty to take sedative drugs. 3. Return to the ward after operation to give ECG monitoring according to the doctor's advice and observe the condition closely.
1.请在术前进行皮肤清洁,如淋浴或擦身等2.术前放松思想,避免情绪紧张,若晚上不能入睡,请告知当班护士,给予服用镇静药物3.术后返回病房遵医嘱给予心电监护,严密观察病情。