Diagnosis of nongonococcal urethritis

summary

When I was 40 years old, I would share my experience in the diagnosis of nongonococcal urethritis.

Diagnosis of nongonococcal urethritis

First, the collection of specimens, generally for urogenital test or brush film, a small number of prostate fluid, semen, joint fluid, or take oviduct, rectal biopsy material, in recent years, with the first section of urine specimen centrifugation, to replace the urethral swab. The swab was inserted into the male urethra for 2-4cm and rubbed with force.

Second, this method is easy to cause urethral injury and secondary infection. Use it carefully. Women need to clean the junction of cervical squamous and columnar epithelium first, and collect cervical specimens with cell brush, which can increase the number of infected cells and is more sensitive than cotton swab. The commonly used culture medium is bovine heart extract or peptone, containing 1% fresh yeast extract, 10-20% animal serum and 0.5% sodium chloride, adding glucose and arginine to promote the growth of MH and Mg, adding urea to metabolize UU, and adding appropriate amount of penicillin to inhibit miscellaneous bacteria.

Third: serological identification: the most commonly used method is agar diffusion method, that is, mycoplasma is inoculated on agar dish. Then put the filter paper soaked with appropriate amount of serum on the agar surface to observe which one can inhibit the growth of Mycoplasma. The advantage of this method is that it can make use of the colonies that initially grow on the agar surface without having to subculture mycoplasma.

matters needing attention

In order to avoid the misdiagnosis of non gonococcal urethritis, we must be careful when carrying out the above examination methods, and strictly treat these examination methods. We need to go to professional medical institutions, otherwise, it will lead to the misdiagnosis of non gonococcal urethritis because of the backward technology of the hospital.