How to use hepatitis C Kit

summary

Hepatitis C is caused by hepatitis C virus (HCV). It is caused by blood transfusion or blood products, hemodialysis, apheresis of plasma and transfusion of blood cells, kidney transplantation, intravenous injection of drugs, sexual transmission, mother to child transmission and other infections. Hepatitis C is widely distributed and is more likely to develop into chronic, liver cirrhosis and liver cancer, ALT and serum bilirubin levels were significantly lower than those of acute hepatitis B. the following is how to use the hepatitis C kit

How to use hepatitis C Kit

The first is that the third generation reagent is more sensitive and specific than the second generation reagent. Taking the anti HCV ELISA reagent of OCD company as an example, the "window period" of the first generation, the second generation and the third generation reagents (that is, the period of early HCV infection and antibody negative but unable to detect) is 150 days, 82 days and 70 days on average respectively. The improvement of the third generation reagents mainly lies in: (1) the proportion of core region antigen is relatively decreased, and the proportion of C33c in NS3 region is correspondingly increased, which optimizes the kit assembly process; ② By using modern gene recombination technology, some important antigens were expressed and purified with better expression and purification system, and the corresponding gene fragments were repositioned. By using various advanced production technologies, the immune activity of coated antigens was stronger, so as to improve the detection sensitivity and reduce the non-specific reaction; ③ NS5 antigen was added.

Second: the antigen used in the first generation of anti HCV IgG kit comes from the non structural region of the virus genome (C100). After screening blood donors, the infection rate of HCV after blood transfusion decreased by more than 80%. But the main defects are as follows: 1) the sensitivity is low, the detection time of antibody is late, and C100 antibody may appear intermittent positive; ② The nonspecific reaction was high.

Third, in addition to the above-mentioned C100 antigen, the second-generation reagent added HCV core region peptide c22-3 and non structural region antigen C33c. Anti-c22-3 and anti-c33c appeared earlier after infection. Moreover, core antigen c22-3 and NS3 C33c antigen were better than C100 in early diagnosis, sensitivity and specificity. The combined application of the above antigens can further improve the detection rate.

matters needing attention

We should pay attention to daily life conditioning, liver nourishing and liver protecting, daily supplement of selenium for liver, which has a protective effect on liver. We can take tihengjian Yanggan tablets containing selenium and Schisandra, which can nourish liver and protect liver, absolutely abstain from alcohol, avoid overwork, maintain a comfortable mood and reasonable nutrition.